免費(fèi)咨詢熱線：
15522676233
產(chǎn)品分類
Product Category相關(guān)文章
Related Articles
簡(jiǎn)要描述：Dulbecco′s Modified Eagle′s Medium - high glucoseWith 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
產(chǎn)品型號(hào)：型號(hào)齊全廠商性質(zhì)：代理商更新時(shí)間：2023-07-11訪 問 量：732Dulbecco′s Modified Eagle′s Medium - high glucose
sterility:sterile-filtered.
form:liquid.
technique(s):cell culture | mammalian: suitable.
impurities:endotoxin, tested.
components:L-glutamine: no;phenol red: yes;sodium pyruvate: yes;NaHCO3: yes;HEPES: no;glucose: high.General description:
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Application:
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
